Quality Control on your behalf: Plasmids and GMO
How many plasmid molecules, genomic DNA and RNA does your preparation contain? What is the gene expression level within the target system? – These questions are becoming more and more important in connection with genetically engineered medications and gene therapy, but are also important in certain areas of research.
Oncoscreen has profound experience in the quantification of the number of copies of genes and the gene expression in prokaryotic and eukaryotic systems. We also perform routine quality control checks on your products, on your behalf.
Quality Control of Plasmid Preparations Copy Number of Plasmids in Transgenic Organisms Gene Expression Analysis of Transgenic Organisms
Quality Control of Plasmid Preparations
A sufficient quality control of plasmid preparations extends beyond the mere determination of the exact plasmid copy number and should for example, also include an exact quantification of contaminants with chromosomal DNA or RNA. Our doctoral molecular biologists are happy to advise you on the spectrum of possibilities and draw up together with you, a quality control procedure adapted to your needs.
Our team remains at your disposal for further questions at all times.
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Copy Number of Plasmids in Transgenic Organisms
With the help of our standardised quality control procedure, we are in a position to reliably quantify the number of copies of genes in arbitrary cells.
To this end, we require from our clients the following:
- a deep-frozen sample with transgenic cells, if possibly of known quantity
- if appropriate, a pre-cut and cleaned plasmid with the target sequence to be used
By using our standardised procedure we can, without further preparation, establish the average number of copies of plasmids per cell.
Dependent on the exact purpose of your investigations, it may be important to also measure the copy number of specific target sequences. Our experienced molecular biologists with doctoral degrees, are more than happy to provide you with competent advice.
Our team remains at your disposal for further questions at all times.
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Gene Expression Analysis of Transgenic Organisms
Studies of gene expression can in principle be carried out in two ways:
- Determination of the number of mRNA molecules per sample (absolute quantification)
- Determination of the genetic expression in relation to a housekeeping gene (relative quantification)
The suitable choice of method depends on exactly what the purpose of your investigation is, and we are happy to advise you on the topic.
For each quantification we require from the customer:
- a cut, cleaned plasmid (on the gel only one band should be visible)
- Information on the size of the plasmid
- the target sequence (which we of course treat with absolute discretion)
- a deep-frozen blood, cell or tissue sample of the transgenic organism
For absolute determination it would also be desirable to have:
- knowledge of the cell number of the sample (depends on the purpose of investigations)
Our team remains at your disposal for further questions at all times.
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